CD4/CD8 (T4/T8 HELPER/SUPPRESS RATIO)

Specimen Required
Draw blood in a lavender EDTA tube. Send 4.0 mL whole blood (1.0 mL minimum) room temperature. Ship within 24 hours of collection.

Rejection Criteria
Refrigerated and frozen samples
Heparinized samples, ACD samples
Clotted specimens

Methodology
Flow Cytometry

Stability
Room Temperature: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Range
Adult >16 years
   T Helper Cell (CD4)   35-66%  
   T Helper Cell (CD4)   443-1471 C/UL  
   T Suppressor Cell (CD8)   9-37%  
   T Suppressor Cell (CD8)   190-832 C/UL  
   T4/T8 Ratio (CD4:CD8)   1.0-3.7 Ratio  
<16 years
   By report  

Performed
Monday-Friday

Turnaround Time3 days
Test CodesCD4/CD8
30-80150
CPT-4 Code (s)
86360
LOINC Codes
T Helper cell (CD4) %   8123-2  
T Helper Cell (CD4) C/UL   24467-3  
T Suppressor Cell (CD8) %   32518-3  
T Supressor Cell (CD8) C/UL   14135-8  
T4/T8 Ratio (CD4:CD8)   54218-3  

Warde
Medical
Laboratory