ADENOVIRUS PCR

Specimen Required
Specimen source required.
Swab specimens (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen-20°C).
Bronchoalveolar lavage/wash in sterile leak-proof container 1.0 mL (0.5 mL minimum).
CSF and body fluid undiluted in sterile leak-proof container. 1.0 mL (0.5 mL minimum).
Nasal aspirated in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in leak proof sterile container. 1.0 mL (0.5 mL minimum).
Blood in EDTA (lavender top) tubes. 2.0 mL (1.0 mL minimum)
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum)
Urine undiluted in sterile leak-proof container. 2.0 mL (1.0 mL minimum)
Bone Marrow in EDTA (lavender top), 1.0 mL (0.5 mL minimum)

Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Rejection Criteria
Rectal swabs preserved in formalin, SAF, or PVA
Urine in Grey-Top (boric acid) urine vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers

Methodology
Polymerase chain reaction (PCR) amplification of a 109 base pair region of the hexon gene and real-time detection using an internal TaqMan detection probe. This method is genus specific and cannot distinguish adenoviral subtypes. The analytical sensitivity of this assay is 200 DNA copies/mL.

Stability
Room Temperature: 3 days; Refrigerated: 2 weeks; Frozen (-20°C): 2 weeks; Frozen (-70°C): 3 months

Reference Range
Negative

Performed
Monday-Saturday

Turnaround Time1-2 days
Test CodesADEPCR
30-92120
CPT-4 Code (s)
87998

Warde
Medical
Laboratory